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P. & M. Tuite (1998) Methods in Microbiology, Vol. 26: Yeast Gene Analysis. Academic Press, New York. , D. Dawson, & T. Stearns (2000) Methods in Yeast Genetics. Cold Spring Harbor Press, New York. Guthrie,C. Fink,editors (1991) Guide to Yeast Genetics and Molecular Biology. Methods in Enzymology, Vol. 194. Academic Press, New York. Labbe, S. J. Thiele (1999) Copper ion inducible and repressible promoter systems in yeast. Methods Enzym. 306: 145-153. , A. J. S. Shah, A. Wach, A. Brachat, P. R. Pringle (1998) Additional modules for versatile and economical PCRbased gene deletion and modification in Saccharonzyces cerevisiae.

T. R. W. A. -H. Lin (1996) Strategies for ProteinPurificationandCharacterization: A LaboratoryCourse Manual. Cold Spring Harbor Press, New York. , F. Chang, N. R. Cross (1993) Negative regulation of FAR1 at the Start of the yeast cell cycle. Genes Dev. 7: 833-843. , J. Konopka, B. Singer-Kruger, M. Zerial, & D. Botstein (1999) Visualization of receptor-mediated endocytosis in yeast. Mol. Biol. Cell 10: 799-817. , L. Riles, M. H. Hegemann (1996) Green fluorescent protein as a marker for gene expression and subcellular localization in budding yeast.

For a thorough reviewof the cellwall reader is referred to Orlean (1997). cell division,mating, and and cellwall biogenesis the CELL WALL COMPOSITION AND SYNTHESIS The major component (80%-90%) of the cell wall ispolysaccharide. P-Glucan is a glucose homopolymer. In Saccharomyces, one finds /3-1,3 straight chains up to 1500 residues long with some p-1,6 branches. These long polymers are intertwined to form microfibrils that are interwoven into the meshwork that makes the basic support structure of the cell wall, much like the steel rods in reinforced concrete.

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Physics Reports vol.57


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