Download e-book for kindle: Differential Geometry: Partial Differential Equations on by Greene R., Yau S.-T. (eds.)

By Greene R., Yau S.-T. (eds.)

ISBN-10: 082181494X

ISBN-13: 9780821814949

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Extra resources for Differential Geometry: Partial Differential Equations on Manifolds, Part 1

Example text

T h e large value of the relative rms fluctuation of 0 . 7 5 % w a s d u e to the fact that fluc­ tuations of a single s a r c o m e r e w e r e considered. Fujime and Y o s h i n o (1978) h a v e investigated b o t h amplitude and time d e p e n d e n c e of force and s a r c o m e r e position fluctuations for an elastic model similar to that of Carlson. In their formulation the x d e p e n d e n c e of forces and rate con­ stants w a s not taken into a c c o u n t , and the resulting p o w e r s p e c t r a w e r e single L o r e n t z i a n s , in contrast to c o m p l e t e expression given by E q .

1973), and the influence of C a on the hindrance of the rotary mobility of myosin h e a d s assembled in thick fila­ m e n t s , w h e r e n o effect w a s found (Mendelson and C h e u n g , 1976). , 1976) and of H M M (Highsmith, 1978) to actin w a s m e a s u r e d . The interaction of actin and the nucleotide binding sites on S-l w a s also followed (Highsmith, 1976). H o w e v e r , w h e n the molecule of interest rotates so slowly that during the fluorescent lifetime the a m o u n t of depolarization is very small, as is most likely the case with the rotational motion of cross-bridges during muscle contraction, the m e t h o d does not provide a useful m e a s u r e m e n t of the ro­ tational diffusion.

This effect r e a c h e d a m a x i m u m at the molar ratio of H M M to actin of 1:6. Binding of S-l m a d e no difference to the relaxation times of actin, w h e r e a s t h e addition of t r o p o m y o s i n m a d e it stiffer, as e x p e c t e d . I s h i w a t a and Fujime (1972) investigated by the s a m e technique the effect of calcium on the flexibility of reconstituted thin filaments, namely the F - a c t i n - t r o p o m y o s i n 2+ troponin complex ( W e b e r and M u r r a y , 1973).

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Differential Geometry: Partial Differential Equations on Manifolds, Part 1 by Greene R., Yau S.-T. (eds.)

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