Read e-book online Current topics in cellular regulation. Vol. 25 PDF

By Bernard L. Horecker, Earl R. Stadtman

ISBN-10: 0121528251

ISBN-13: 9780121528256

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S * forward process of F (sensitivity = rjs)). 2. Forward process of F—*rate of F (sensitivity = R = 1, since F is nonequilibrium in this example). 3. Rate of F ~ * n e t flux / (sensitivity = 1 + C//). 6 Therefore, applying the "product r u l e " [Eq. , the forward and reverse reactions). (s)> v * a interaction with F, = rjs)(l + C/J) 5 i(s)> v * a interaction with C, = rfs^(—C/J) (see Table I for the functions). The overall effect is then obtained by the addition rule [Eq. (9)], so that The situation becomes a little more complex if one of the reactions of the cycle is near-equilibrium.

A constant) so that, by analogy with a conserved pool, the equation can be approxi­ mated by the power equation, This enables all sums and differences of concentrations and fluxes to be expressed as products, raised to a power, thereby making subsequent manipulations very straightforward. E. Effect of Near-Equilibrium Nature of a Reaction on the Sensitivity to Catalystic (Allosteric) and Mass-Action Regulators In Section III,C (Fig. 2), the overall sensitivity of a flux/to a regulator X was derived in terms of the intrinsic sensitivities of the component reactions [Eq.

1 Since the value of sE has already been derived, this relationship allows sE to be derived without manipulating the power equations for the system. 13 Functions such as sE and sE can be measured by "titrating" fluxes with specific (or relatively specific) inhibitors or activators of the regula­ tory reactions [see, for example, the investigations of Groen et al (l 7) on the control of mitochondrial respiration]. Such investigations can give useful information about the potential of each regulatory site in situ, but at least two major problems may arise if the approach is used uncritically.

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Current topics in cellular regulation. Vol. 25 by Bernard L. Horecker, Earl R. Stadtman


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