By R. H. Quarles, R. G. Farrer, S. H. Yim (auth.), Bernhard H. J. Juurlink, Richard M. Devon, J. Ronald Doucette, Adil J. Nazarali, David J. Schreyer, Valerie M. K. Verge (eds.)
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Additional info for Cell Biology and Pathology of Myelin: Evolving Biological Concepts and Therapeutic Approaches
MAG is enriched in periaxonal regions of myelin fibers in both CNS (A) and PNS (B). Some fibers in the PNS have darker and thicker bands of staining (B). In longitudinal orientation these are resolved as paranodal regions (c. arrowheads) or Schmidt-Lanterman incisures (C, arrows). In ultrathin cryosections of peripheral nerve (D), MAG immunogold labeling is enriched in Schmidt-Lanterman incisures (SL) and periaxonal membranes (D, arrowheads). but not in compact myelin. 25 Ilm. Reproduced with permission from Trapp et al..
MOG epitopes were detected with Texas Red-conjugated goat anti-rabbit IgG (I :200), and p-tubulin was identified with fluorescein-conjugated goat anti-mouse IgG (J:IOO)(3rC, 3 hr). Anti-MOGI-125 staining gave strong reactivity for surface staining with both unpermeabilized and permeabilized HEKMOG cells (a, b, respectively). In contrast, intense staining with MOGI54-169, MOG 198-218, or p-tubulin antisera (I : 100) required saponin permeabilization ofHEKMOG cells (f, g, h, respectively); no surface reactivity was found with unpermeabilized HEKMOG cells (b, c, d).
B-expressing cells based on glycolipid-glycolipid interaction. J Bioi Chem 266: 17552-17558. Kojima N. Hakemori S (1989): Specific interaction between gangliotriaosylceramide and sialosyllactosy1ceramide (GM3) as a basis for specific cellular recognition between lymphoma and melanoma cells. J Bioi Chem 264: 20159-20162. Korngurth S. Anderson L (1965): Localization of a basic protein of various species with the aid of fluorescence and electron microscopy. J Cell Bioi 26: 157-166. Larocca IN. Ledecn RW.
Cell Biology and Pathology of Myelin: Evolving Biological Concepts and Therapeutic Approaches by R. H. Quarles, R. G. Farrer, S. H. Yim (auth.), Bernhard H. J. Juurlink, Richard M. Devon, J. Ronald Doucette, Adil J. Nazarali, David J. Schreyer, Valerie M. K. Verge (eds.)