By Elisa Michelini, Luca Cevenini, Laura Mezzanotte, Aldo Roda (auth.), Preston B. Rich, Christelle Douillet (eds.)
Through the examine and alertness of bioluminescence, scientists have painstakingly harnessed a strong device that allows us to hunt a deeper realizing of the advanced mechanisms underpinning such a lot of very important biologic structures. during this absolutely revised and up-to-date moment version of Bioluminescence: equipment and Protocols, specialist researchers give a contribution a readable and utilitarian compilation of the most recent and so much leading edge options that experience emerged during this speedily increasing and gradually various box together with ways to determine mobile trafficking, protein-protein interactions, intracellular signaling, and apoptosis. additionally starting up the prospect to imagine and quantify organic mechanisms in real-time and in in vivo settings, the amount additionally describes the in vivo examine of bacterial or viral infections, transplanted cells, stem cells proliferation, vascular circulation, and tumors. Written within the hugely winning Methods in Molecular Biology™ sequence layout, chapters contain short introductions to their respective themes, lists of the required fabrics, apparatus, and reagents, step by step, conveniently reproducible laboratory protocols, and notes on troubleshooting and fending off recognized pitfalls.
Authoritative and state-of-the-art, Bioluminescence: equipment and Protocols, moment variation provides protocols which are designated sufficient to be and tailored through medical groups who've no past services in bioluminescence that allows you to foster the possibly various breakthroughs and new functions from easy to utilized technological know-how and medication that needs to stay developed.
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Additional info for Bioluminescence: Methods and Protocols
Contag, C. , and Negrin, R. S. (2003) Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging. Blood 101, 640–648. 3. Sweeney, T. , Tucker, A. , Olomu, A. , Negrin, R. , and Contag, C. H. (1999) Visualizing the kinetics of tumor-cell clearance in living animals. Proc Natl Acad Sci USA 96, 12044–12049. 4. , Bollinger, R. , Braasch, D. , Corey, D. , Antich, P. , and Validation of Bioluminescent Imaging Techniques 5. 6. 7. 8. 9. 10. 11. 12. 13. Mason, R.
Colon carcinoma xenograft model. Cancer Biol Ther 2, 196–202. , Chan, D. , Gambhir, S. , Giaccia, A. , and Graves, E. E. (2007) Oxygen sensitivity of reporter genes: implications for preclinical imaging of tumor hypoxia. Mol Imaging Biol 6, 219–228. Karam, J. , Benaim, E. , Sagalowsky, A. , Mason, R. , and Hsieh, J. -T. (2007) The use of histone deacetylase inhibitor FK228 and DNA hypomethylation agent 5-Azacytidine in human bladder cancer therapy. Int J Cancer 120, 1795–1802. Klerk, C. , Overmeer, R.
Light detection is strongest from subcutaneous tumor sites, although in this case caliper measurements may be just as effective and cheaper for simple tumor volume assessment. However, BLI is particularly effective for low tumor burdens, and indeed, subpalpable volumes can be detected and quantified (Fig. 1). For large tumors, self-absorption and scatter of light can bias apparent relative tumor volume. Planar BLI appears to accurately reflect the volume of small tumors, but becomes less linear for larger tumors although continuing to increase monotonically (12, 13).
Bioluminescence: Methods and Protocols by Elisa Michelini, Luca Cevenini, Laura Mezzanotte, Aldo Roda (auth.), Preston B. Rich, Christelle Douillet (eds.)