By Jean Brachet
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Extra info for Biology of Acetabularia
A) The RNA's extracted from whole plants. b) The RNA's extracted from anucleate fragments, 5 days after surgery. c) The ribosomes from a total homogenate (whole plants). d) The RNA extracted from the labeled 30 s ribosomes; total Acetabularia RNA has been added as a carrier. e) The RNA extracted from the labeled 50 s ribosomes; total Acetabularia RNA has been added as a carrier. o-o-o : radioactivity #-·-· : absorbance 31 MICHEL JANOWSKI AND SILVANO BONOTTO 0 10 20 30 40 Figure 4. 4 day-incorporation of H -uridine (2 yc/ml) into RNA's of Acetabularia mediterranea.
30 BIOLOGY OF ACETABULARIA a 25 23 17 16 Y */ *v III ^ 5 o 0 i 10 25 23 16 1 20 —T 10 1 20 s'o 1 — 30 40 r 30 40 f r a c t ion Figure 3. 30 min-incorporation of H -uridine (20 uc/ml) into the RNATs and ribosomes of Acetabularia mediterranea. a) The RNA's extracted from whole plants. b) The RNA's extracted from anucleate fragments, 5 days after surgery. c) The ribosomes from a total homogenate (whole plants). d) The RNA extracted from the labeled 30 s ribosomes; total Acetabularia RNA has been added as a carrier.
Buoyant density increases to the right. 704 Fig. 2: Chloroplast fraction DNA 48 BIOLOGY OF ACETABULARIA Renatured Fig. 3: DNA Chloroplast DNA (a) Heat-denatured and quick-cooled. (b) Heat-denatured and renatured at 70° C. See text. 49 GREEN, BURTON, HEILPORN, AND LIMBOSCH 1715 Fig. 4: Mitochondrial fraction DNA 50 BIOLOGY OF ACETABULARIA Fig. 5: C y s t DNA from n e w l y - r i p e n e d caps. 1697 Fig. 6: DNA from caps stored at 10°C then exposed to light for 3 days at 20°C. 51 GREEN, BURTON, HEILPORN, AND LIMBOSCH Plate 1.
Biology of Acetabularia by Jean Brachet