By J. B. C. Findlay, W. H. Evans
Every one animal and plant mobile should be built of a minimum of ten special varieties of membrane whose isolation and characterization are actually possible utilizing the wide-ranging innovations provided during this booklet. either newly constructed and extra proven equipment for the research of proteins and lipids and for the research in their topography are defined intimately. additionally supplied are methods for the reconstitution and characterization of membrane platforms. specific consciousness has been paid to immunological techniques for separating and reading membranes and their parts and for screening expression structures in molecular cloning. these biophysical and physico-chemical concepts that have additional a lot to our knowing of membrane constitution and serve as also are incorporated. The textual content accordingly presents a entire choice of sleek recommendations and ways utilized in the research of organic membranes.
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Extra resources for Biological membranes: a practical approach
Postmortem human heart sample showing gross hypertrophy and reduction in left ventricular chamber size (A) associated with myocyte hypertrophy, myofiber disarray and interstitial fibrosis (B). Postmortem heart sample form mouse expressing the Arg403Gln mutation in the myosin heavy chain gene, showing myocardial hypertrophy and left atrial enlargement (C) associated with typical histopathological features of HCM (D). LA, left atrium; LV, left ventricle; RV, right ventricle. 4. Inhibition of calcineurin signaling prevents the development of DCM in activated calcineurinexpressing transgenic mice.
From Molkentin JD, Lu JR, Antos CL et al A calcineurin-dependent transcriptional pathway for cardiac hypertrophy. Cell 1998; 93:215–28, with permission. 1. Genetic definition of the molecular basis for cardiomyopathy in two families. Pedigrees indicated sex (circles denote women, boxes denote men) and clinical status (solid fill, affected; open, unaffected, hatched uncertain status). Genotypes are provided: +mutation present; −mutation absent. (A) Sudden death in multiple individuals (I-1, II-5, -6, -8) prompted clinical evaluation of surviving family members.
Genes with similar expression patterns are clustered together in 58 different clusters, and the clusters were arranged by nearest similarity to other clusters. Reproduced with permission from Stanton LW, Garrard LJ, Damm D et al. Altered patterns of gene expression in response to myocardial infarction. Circ Res 2000; 86:939–45. 2. Comparison of mouse and human HCM. Postmortem human heart sample showing gross hypertrophy and reduction in left ventricular chamber size (A) associated with myocyte hypertrophy, myofiber disarray and interstitial fibrosis (B).
Biological membranes: a practical approach by J. B. C. Findlay, W. H. Evans