Get Bioconjugation Protocols: Strategies and Methods (Methods in PDF

By Christof M. Niemeyer

ISBN-10: 1588290980

ISBN-13: 9781588290984

ISBN-10: 1592598137

ISBN-13: 9781592598137

Professional laboratorians replace the vintage bioconjugation tools and introduce priceless new strategies that transcend natural biomedical purposes to incorporate parts from complicated natural synthesis, molecular biology, and fabrics technological know-how. those with no trouble reproducible tools hide the coaching of protein conjugates utilizing covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates utilizing various labeling options, and techniques to semisynthetic conjugates of proteins. extra chapters tackle the biofunctionalization of inorganic surfaces, together with the on-chip synthesis of peptide nucleic acids to generate microarrays for the high-throughput research of RNA and DNA, gold nanaoparticles, and carbon nanotube probes for atomic strength microscopy.

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Additional resources for Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology Vol 283)

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Anderson, D. , et al. (1995) Regional differences in constitutive and induced ICAM-1 expression in vivo. Am. J. Physiol. 269, H1955–H1964. 11. , Rijken, D. , and van Berkel, T. J. (1988) Characterization of the interaction in vivo of tissue-type plasminogen activator with liver cells. J. Biol. Chem. 263, 18,220–18,224. Smart Polymer–Streptavidin Conjugates 37 3 Smart Polymer–Streptavidin Conjugates Patrick S. Stayton, Zhongli Ding, and Allan S. Hoffman Summary The conjugation of stimuli-responsive, or “smart,” polymers to streptavidin is described.

1990) Thermally on-off switching polymers for drug permeation and release. J. Control Release 11, 255–265. 6. , and Bahr, U. (1994) Synthesis and characterization of a highly uniform, thermosensitive affinity macroligand precursor suitable for carbodiimide coupling. Bioseparation 4, 353–367. 7. 7 Bromberg, L. and Levin, G. (1998) Poly(amino acid)-b-poly(N, N-diethylacrylamide)-b-poly(amino acid) conjugates of well-defined structure. Bioconj. Chem. 9, 40–49. 8. , Tan, P. , and Stayton, P. S. (1995) Site-directed mutagenesis studies of the high affinity streptavidin-biotin complex: contributions of tryptophan residues 79, 108, and 120.

Closed arrowheads show a triple FITC+Alexa Fluor 350+Texas Red-labeled particle, located to the cell surface. Open arrowheads show a double FITC+Texas Red-labeled particle, which indicates that the targeting antibody was not degraded after internalization within the cell. The arrow shows single FITC-labeled particles, indicating that the targeting antibody in the internalized particles has been degraded. 2. 1. BIODISTRIBUTION AND PULMONARY TARGETING OF tPA CONJUGATED WITH ANTI-ICAM Experiments with tPA conjugated with an ICAM-1 monoclonal antibody illustrate analysis of vascular immunotargeting in vivo.

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Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology Vol 283) by Christof M. Niemeyer

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