Read e-book online Atomic Force Microscopy in Cell Biology, Volume 68 PDF

By Bhanu P. Jena

ISBN-10: 0125441711

ISBN-13: 9780125441711

This is often the 1st publication to hide the background, constitution, and alertness of atomic strength microscopy in cellphone biology. awarded within the transparent, well-illustrated variety of the Methods in mobile Biology sequence, it introduces the AFM to its readers and permits them to faucet the facility and scope of this know-how to extra their very own study. a realistic laboratory advisor to be used of the atomic strength and photonic strength microscopes, it offers up to date know-how and techniques in strength spectroscopy. it's also a accomplished and easy-to-follow useful laboratory advisor for using the AFM and PFM in organic study.

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Extra resources for Atomic Force Microscopy in Cell Biology, Volume 68

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The reaction coordinate of unfolding is imposed by the direction of pulling, and in this way unfolding events occurring in a single protein can be examined. Each peak is attributed to a breakage of a main stabilizing connection of a folded protein structure. Recombinant DNA techniques were also used to construct tandem repeats from one single spectrin domain for such experiments. The method extends the monomer (R16) at both ends so 1. Local Probe Techniques 17 Fig. 11 Unfolding by AFM of an artificial spectrin polypeptide chain fixed on a gold surface by cysteines.

In this situation, a possible approach is to find a natural system where a certain type of channel is important for the function. Such a highly specialized system is the mechanoelectrical transduction system of the hair cells of the inner ear. Cochlear hair cells of the inner ear are responsible for both detecting sound and encoding the magnitude and time course of an acoustic stimulus such as an electric receptor potential, which is generated by a still unknown interaction of cellular components.

And H¨orber, J. K. H. (2000). J. Cell Biol. 148, 997. , Stelzer, E. H. , and H¨orber, J. K. H. (1999). Microsc. Res. Tech. 44, 378. , Fernandez, J. , and Gaub, H. E. (1997). Science 276, 1109. Ruppersberg, J. , H¨orber, J. K. , and Binnig, G. (1989). FEBS Lett. 257, 460. Saffman, P. , and Delbr¨uck, M. (1975). Proc. Natl. Acad. Sci. USA 72, 3111. Schneider, S. , Kumudesh, C. , Geibel, J. , and Jena, B. P. (1997). PNAS 94, 316. , and Ikonen, E. (1997). Nature 387, 569. Smith, D. P. , and Binnig, G.

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Atomic Force Microscopy in Cell Biology, Volume 68 by Bhanu P. Jena

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