Download PDF by Jürgen Hescheler, Erhard Hofer: Adult and Pluripotent Stem Cells: Potential for Regenerative

By Jürgen Hescheler, Erhard Hofer

ISBN-10: 9401786569

ISBN-13: 9789401786560

ISBN-10: 9401786577

ISBN-13: 9789401786577

There is rarely a space of study constructing so quick and elevating such a lot of grants as stem phone learn. grownup, embryonic and lately to be had caused pluripotent stem cells not just foster our realizing of differentiation of endo-, ecto- and mesodermal lineages to all organs of the physique, yet prime nourish the wish that cells grown in tradition can be utilized for regeneration of diseased organs equivalent to the center broken by means of myocardial infarction. This publication specializes in views of stem cells for regenerative treatment of cardiovascular illnesses. according to the EC consortium INELPY, it studies the sector and disseminates significant results of this undertaking. hence it introduces the reader to this attention-grabbing sector of study and contains very fresh findings fascinating to the specialist, spanning the sector from bench to bedside. The compilation of contributions is exclusive as there's but no related accomplished evaluation combining stem mobile examine with preclinical and medical overview in addition to engineering of tissue patches for transplantation. As such will probably be a useful resource of data for all researchers within the stem cellphone and tissue regeneration box together with bioengineers in addition to for all clinicians attracted to regenerative treatments, in particular for ischemic cardiomyopathies.

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Extra resources for Adult and Pluripotent Stem Cells: Potential for Regenerative Medicine of the Cardiovascular System

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2000; Itzhaki-Alfia et al. 2009) and non-enzymatic approaches (Smith et al. 2007; Messina et al. 2004). Using the enzymatic approach, researchers used various enzymes to dissociate the cells from the myocardial specimens. Then, stem cells were isolated from the dissociated cells with a magnetic cell sorter using antibodies against stem cell markers, such as c-kit (Bearzi et al. 2007). However, the cell yield was low and required several months to grow a sufficient number of cells for potential therapeutic applications (Argentin et al.

2009). 3 Isolation methods used to dissociate human heart tissue Living/ Technique used Researchers Percentage of tissue that yielded dead cell ratios progenitor cells (Shneyvays No data No data RDB proteolytic enzyme et al. 1998) (Institute of Biology, Nes-Ziona,Israel) Collagenase (Bearzi et al. 8 of 12 No data 2007) Explanted technique (Bearzi et al. 46 of 70 No data 2007) Explanted technique (Smith et al. 69 of 70 No data 2007) (Parker et al. 25 % and (Itzhaki-Alfia 249 of 249 95–98 % dispase II et al.

C. Bearzi (Bearzi et al. 2007) from the Anversa group was the first to report that human cardiac stem cells (hCSCs), isolated from human biopsies, were positive for c-kit, a stem cell growth factor receptor, also known as CD117. The c-kit receptor was originally detected in murine HSCs with ability to restore bone marrow in irradiated recipients (Orlic et al. 1993). Anversa et al. showed a major contribution of c-kit progenitor cells to cardiomyogenesis during embryonic development (Ferreira-Martins et al.

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Adult and Pluripotent Stem Cells: Potential for Regenerative Medicine of the Cardiovascular System by Jürgen Hescheler, Erhard Hofer


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