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By Edelmann G.

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1. Imprinted chromosomal regions in the mouse genome. Robertsonian and reciprocal translocations have been used to obtain evidence of imprinting for 11 different regions on eight autosomal chromosomes. uk), from which this simplified summary figure was adapted. For each imprinted region (in gray), the main phenotypic consequences of maternal disomy and paternal disomy are indicated to the left and the right of the chromosome, respectively. Additionally, the figure depicts the mouse X chromosome, which has an aberrant extra-embryonic phenotype associated with maternal disomy (31).

4. Lysing buffer: 1 mg/mL proteinase K (Boehringer, Mannheim), 1% SDS, 250 mM EDTA. 5. Stocking buffer: 10 mM Tris-HCl, pH 8; 50 mM EDTA; 1 M NaCl. 6. 10X TBE buffer: 890 mM Tris, 890 mM boric acid, 20 mM EDTA, pH 8 (used diluted 1:20 for electrophoresis). 7. 200 mM PMSF dissolved in isopropyl alcohol (stored –20°C). 8. 10 mg/mL EtBr. 9. 0. 10. 2% Low-melting point agarose (FMC BioProducts, Rockland, ME) prepared on KPSS buffer w/o spermine, spermidine, and PMSF. 11. 5X TBE buffer. 12. YAC telomere-specific probe (see Note 2).

Bosma, A. , et al. (1996) Report on the progress of standardization of the G-banded canine (Canis familiaris) karyotype. Chrom. Res. 4, 306–309. 6. Bowling, A. , Chowdhary, B. , et al. (Committee). ISCNH. (1997) International system for cytogenetic nomenclature of the domestic horse. Chrom. Res. 5, 433–443. 7. , coordinator. (1988) Committee for the standardized karyotype of the domestic pig. Standard karyotype of the domestic pig. Hereditas 109, 151–157. 8. Popescu, C. , eds. Texas Nomenclature.

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3-Folds in P5 of degree 12of degree 12 by Edelmann G.


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